File Name: C:\Program Files\Bio-Rad\Bio-Plex Manager 5.0\users\Craig Miller\Results\Saliva IgA Luitpold d71 080213.rbx
Analyte: IgA (63)
Acquisition Date: 02-Aug-2013, 01:37 PM
Reader Serial Number: LX10008241401
Plate ID: 
RP1 PMT (Volts): 647.62
RP1 Target: 17026
Analyte Type Well Outlier Description FI FI - Bkgd Std Dev Std Err %CV Obs Conc Exp Conc (Obs/Exp) * 100 Conc in Range Bead Count Sampling Errors
IgA (63) B A1,B1,C1,D1 0 63.5 63.5 3.34 1.67 5.26
IgA (63) S1 E1,F1 2 Standard 1 10064.5 10001 0 0 0 430.89 500 86 430.89
IgA (63) S2 G1,H1 0 Standard 2 9491.8 9428.3 54.8 38.75 0.58 286.37 250 115 286.37
IgA (63) S3 A2,B2 0 Standard 3 7274 7210.5 65.76 46.5 0.9 121.71 125 97 121.71
IgA (63) S4 C2,D2 0 Standard 4 5022 4958.5 165.46 117 3.29 62.59 62.5 100 62.59
IgA (63) S5 E2,F2 0 Standard 5 2987.8 2924.3 20.15 14.25 0.67 30.37 31.25 97 30.37
IgA (63) S6 G2,H2 0 Standard 6 1786.5 1723 48.79 34.5 2.73 15.89 15.63 102 15.89
IgA (63) S7 A3,B3 0 Standard 7 1045 981.5 18.38 13 1.76 8.26 7.81 106 8.26
IgA (63) S8 C3,D3 2 Standard 8 542 478.5 0 0 0 3.75 3.91 96 3.75
IgA (63) X1 E3,F3 0 4664 7444.8 7381.3 19.45 13.75 0.26 128260.39 128260.39
IgA (63) X2 G3,H3 0 4656 6650.8 6587.3 20.86 14.75 0.31 101101.54 101101.54
IgA (63) X3 A4,B4 0 4661 5367.3 5303.8 126.22 89.25 2.35 69466.65 69466.65
IgA (63) X4 C4,D4 0 4331 6673.5 6610 231.22 163.5 3.46 101777.23 101777.23
IgA (63) X5 E4,F4 0 4326 1181.8 1118.3 4.6 3.25 0.39 9590.03 9590.03
IgA (63) X6 G4,H4 0 4651 11470.5 11407 142.13 100.5 1.24 OOR > ***
IgA (63) X7 A5,B5 0 4654 920 856.5 18.38 13 2 7084.59 7084.59
IgA (63) X8 C5,D5 0 4655 1274 1210.5 31.11 22 2.44 10506.2 10506.2
IgA (63) X9 E5,F5 0 4659 3022.8 2959.3 20.15 14.25 0.67 30836.01 30836.01
IgA (63) X10 G5,H5 0 4662 3966 3902.5 280.01 198 7.06 44392.01 44392.01
IgA (63) X11 A6,B6 0 4666 1892 1828.5 15.56 11 0.82 17052.02 17052.02
IgA (63) X12 C6,D6 0 4667 6135.3 6071.8 90.86 64.25 1.48 87015.21 87015.21
IgA (63) X13 E6,F6 0 4670 6135.5 6072 98.29 69.5 1.6 87021.53 87021.53
IgA (63) X14 G6,H6 0 4671 9294.3 9230.8 74.6 52.75 0.8 257680.14 257680.14
Analyte Type Well Outlier Description FI FI - Bkgd Std Dev Std Err %CV Obs Conc Exp Conc (Obs/Exp) * 100 Conc in Range Bead Count Sampling Errors
IgA (63) B A1 0 62 62 131
IgA (63) B B1 0 59.5 59.5 136
IgA (63) B C1 0 66.5 66.5 154
IgA (63) B D1 0 66 66 141
IgA (63) S1 E1 0 10064.5 10001 430.89 500 86 430.89 124
IgA (63) S1 F1 1 11518.5 11455 500 120
IgA (63) S2 G1 0 9530.5 9467 292.8 250 117 292.8 130
IgA (63) S2 H1 0 9453 9389.5 280.23 250 112 280.23 127
IgA (63) S3 A2 0 7320.5 7257 123.45 125 99 123.45 128
IgA (63) S3 B2 0 7227.5 7164 120 125 96 120 120
IgA (63) S4 C2 0 5139 5075.5 64.86 62.5 104 64.86 139
IgA (63) S4 D2 0 4905 4841.5 60.38 62.5 97 60.38 138
IgA (63) S5 E2 0 2973.5 2910 30.18 31.25 97 30.18 154
IgA (63) S5 F2 0 3002 2938.5 30.56 31.25 98 30.56 144
IgA (63) S6 G2 0 1752 1688.5 15.51 15.63 99 15.51 139
IgA (63) S6 H2 0 1821 1757.5 16.26 15.63 104 16.26 162
IgA (63) S7 A3 0 1058 994.5 8.39 7.81 107 8.39 125
IgA (63) S7 B3 0 1032 968.5 8.14 7.81 104 8.14 157
IgA (63) S8 C3 1 534 470.5 3.91 129
IgA (63) S8 D3 0 542 478.5 3.75 3.91 96 3.75 140
IgA (63) X1 E3 0 7431 7367.5 127715.54 127715.54 144
IgA (63) X1 F3 0 7458.5 7395 128808.41 128808.41 178
IgA (63) X2 G3 0 6636 6572.5 100666.15 100666.15 123
IgA (63) X2 H3 0 6665.5 6602 101539.05 101539.05 116
IgA (63) X3 A4 0 5456.5 5393 71335.36 71335.36 128
IgA (63) X3 B4 0 5278 5214.5 67636.88 67636.88 137
IgA (63) X4 C4 0 6510 6446.5 97030.29 97030.29 136
IgA (63) X4 D4 0 6837 6773.5 106787.74 106787.74 132
IgA (63) X5 E4 0 1185 1121.5 9622.03 9622.03 123
IgA (63) X5 F4 0 1178.5 1115 9558.06 9558.06 114
IgA (63) X6 G4 0 11370 11306.5 OOR > *** 109
IgA (63) X6 H4 0 11571 11507.5 OOR > *** 117
IgA (63) X7 A5 0 933 869.5 7205.57 7205.57 103
IgA (63) X7 B5 0 907 843.5 6963.99 6963.99 122
IgA (63) X8 C5 0 1296 1232.5 10727.12 10727.12 100
IgA (63) X8 D5 0 1252 1188.5 10286.2 10286.2 143
IgA (63) X9 E5 0 3008.5 2945 30646.82 30646.82 132
IgA (63) X9 F5 0 3037 2973.5 31025.62 31025.62 125
IgA (63) X10 G5 0 3768 3704.5 41364.73 41364.73 100
IgA (63) X10 H5 0 4164 4100.5 47528.22 47528.22 123
IgA (63) X11 A6 0 1881 1817.5 16929.49 16929.49 146
IgA (63) X11 B6 0 1903 1839.5 17174.77 17174.77 109
IgA (63) X12 C6 0 6199.5 6136 88654.26 88654.26 106
IgA (63) X12 D6 0 6071 6007.5 85405.87 85405.87 123
IgA (63) X13 E6 0 6205 6141.5 88795.99 88795.99 137
IgA (63) X13 F6 0 6066 6002.5 85281.85 85281.85 104
IgA (63) X14 G6 0 9241.5 9178 250979.64 250979.64 106
IgA (63) X14 H6 0 9347 9283.5 264751.62 264751.62 118
Sampling Errors: 1 - Low bead #, 2 - Agg beads, 3 - Classify %, 4 - Region selection, 5 - Platform temperature
***Value not available / --- = Designated as an outlier
*Value extrapolated beyond standard range
OOR = Out of Range / OOR> = Out of Range Above  / OOR< = Out of Range Below
Exp Conc = Expected Concentration  /  Obs Conc = Observed  Concentration
Conc in Range = Unknown sample concentrations within range where standards recovery is 70-130%
Regression Type: Logistic - 5PL
Std. Curve: FI = -63.499 + (10720.6 + 63.499) / ((1 + (Conc / 134.326)^-1.64985))^0.506246
FitProb. = 0.5666, ResVar. = 0.6761